Chirality pairing recognition, a unique reaction forming spiral alkaloids from amino acids stereoselectively in one-pot
Abstract
A novel chirality pairing recognition was found between D-and L-amino acid derivatives. Novel spiral alkaloids formed in the recognition reaction. Possible mechanism was proposed for the stereoselective and chemoselective reactions.Keywords
chirality pairing recognition reaction spiral alkaloid amino acid derivativesIntroduction
Sexual selectivity and recognition are the important characteristics of life in evolutions in nature since "sex" means re-combination between two "sexual units", and "recognition" means one unit can select the most suitable one for its re-productions, which looks like a male needs a female in a family. Although "molecular sex" was first used in nucleic acids hybridize studies1, and "molecular recognition" has been widely used in chiral compound separations, which via space match each other, however, they are physical behaviors.2 In this report, we show the first chemical behavior example of chirality pairing recognition reaction or molecular sex recognition reactions where D-(+)-amino acid derivative could selectively react with the corresponding L-(–)-amino acid analog to form single product combined by D-and L-amino derivative (D-L) instead of mixtures of D-D and L-L products. Novel spiral alkaloid with unique sketch formed in the one-pot reactions.
Results and Discussion
Our initial attempt at synthesizing chiral compound 3 by reacting an oxalaldehyde with a tryptophan methyl ester (1) in CH2Cl2 did not yield the desired product 3 (Fig. 1). The major compound (60% yield) obtained had neither the quaternary C-4'a at 108.3 ppm nor C-9'a at 130.8 ppm, but had one new quaternary 13C at 64.7 and one new tertiary 13C at 88.3 ppm. This indicated that one C=C of the second indole moiety became a C-C bond. A new condensation reaction happened in the procedure after normal Pictet-Spengler reaction in the first condensations.4 H-H COSY, HMBC and HSQC experimental results exhibited that its planar structure as 4. The key NOE between the H-3/H-9' and H-1/H-1' in ROESY experiments suggested that the major product have the 4a stereochemistry. Moreover, the experimental high resolution MS data (459.2022, [M + H]+) agrees well with the calculated M+ of 458.1954 (Fig. 1). Similarly, the minor product had the structure of 4b from cis-2.
Possible structures in the new condensation reactions
We also used amines (5, 6, 9 and 10) and esters (7 and 8) in the study. When 5 was used as the starting material, compound 11 were obtained with about 47% yield. Similarly, 12 from 6, 13 from 7, 14 from 8 and 15 from 9 were obtained, respectively. The results are summarized below. It was found that the presence of a strong electron-withdrawing group of –OH on the indole ring of 10 inhibited the reaction to 16, while the presence of CH3 on 9 promoted the reaction to 15.
A mechanism for the transformation is proposed (Fig. 2) whereby after the second Schiff base (17) is formed, N-2' can chelate with an H+ to form the first five-membered ring (C-1'– C-4'a formation, 18), then the positive charge can transfer to C-9'a. N-2 in 19 immediately connected to C-9'a after loss of a H+ via a concerned procedure.5 The key requirement in the cycloaddition reactions is that a proton must be present on the indole N atom. Thus, if this proton was replaced by a methyl group as in 20 and 21, the condensation cannot take place. This prediction is recorded when 20 or 21 was used in the condensations.
Proposed mechanisms for formation of 4 from intermediate 17
The unexpected molecular chirality pairing recognition was observed when a (D)-amino acid derivative was mixed with an equal molar of the corresponding (L)-amino acid derivative. For example, D-(+)-1 reacted with L-(–)-1, only one major product formed. This product can be separated as two compounds using chiral column. Their 1H and 13C NMR were the same, and the determined optical rotation for 22 was +187.3, while the ent-22 had –189.2 in chloroform. It exhibited that the separated two products were the enantiomers. Its planar structure was well established as 22 using 13C NMR and HMBC spectra (see Electronic Supplementary Material for more details). Its relative configuration was identified using ROESY experiments. The key interactions between the H-3 and H-9'a, and H-1'and H-3' showed it having the relative configuration of 22. Further absolute configuration determination was performed by comparing their optical rotation and circular dichroism to those obtained via quantum theory. Other pairs of substrates, D-(+)-8 and L-(–)-8, D-(+)-7 and L-(–)-7, D-(+)-6 and L-(–)-6, were used in the reactions, only one major L-D product formed, there was no D-D, or L-L products in the reactions. In the L-D products, each pair of them was separated into two enantiomers. Their optical rotations for the enantiomers of 23, 24 and 25 were +150.3, +127.2 and +166.7. The recorded optical rotations for ent-23, ent-24 and ent-25 were –148.5, –130.2 and –170.3, respectively. The results are summarized in Table 1.
Chirality pairing recognition reactions in presence of CHOCHO
Furthermore, as shown in Fig. 3 below, the TLC spots and HPLC retention times of the different products are examined. Clearly the differences among the D-D and D-L products are obvious. For example, the Rf value of the D-D product 4a from D-(+)-1 was about 0.8, while that of D-L product derived from D-(+)-1 and L-(–)-1 was about 0.45. The minor product obtained in the same reaction 4b had about 0.30 of Rf value. When the three compounds were mixed at c point, the three compounds were well separated, and they exhibited the same Rf values as the standard point in the left (1#). The different Rf values between the D-D products 13 (derived from D-(+)-7 (2#)) and D-L products 23 (derived from D-(+)-7 and L-(–)-7)) were also obvious. Similarly, the D-D product 14 from D-(+)-8 had different Rf value from that of 24, which was derived from D-(+)-8 and L-(–)-8. The Rf values of 12 (D-D product from D-(+)-6) was the same as that of 25 which derived from D-(+)-6 and L-(–)-6 in TLC experiments. It looks like that this does not agree with the obtained results. However, they had different retention time (RT) in HPLC using chiral column (4#). For example, the D-D product 12 had about 17.3 minute, L-L product had very close RT of 17.52 minutes. But the D-L product 25 (include its ent-25, L-D product) had 13.7 or 16.1 minute. The experiments confirmed the products formed in the reactions of the D-and L-amino acid analogue mixtures are different from the use of D-or L-amino acid analogue.
TLC analyses (1# to 3#), spot a: pure major product (D-D, or L-L) from D-or L-amino acid derivatives; a1: pure minor product; b: products in solution of D-and L-amino acid derivatives; c: mixed a (and a1 in 2#) and b (ethyl acetate:petrol ether = 1:1). Mixtures of ethyl acetate/petrol ether with 1.5/1 were used in 1# and 1/1 in 2#. CHCl3 and methanol (60:1) was used in 3#. In 4#, case Ⅰ is the retention time (RT, minute) of reaction product of D-methyltrypotamine in HPLC using chiral column; case Ⅱ is the RT for retention time for reaction product of L-methyltrypotamine. Case Ⅲ is the RT for mixture of D-and L-methyltrypotamine products, and their RTs in HPLC are different from those in cases Ⅰ and Ⅱ (their Rf values in TLC are the same under lab conditions).
![]() |
This special reaction should belong to chirality recognition reaction. However, it may be called as chirality pairing recognition reaction, or molecular sex recognition reaction if D-(+)-or L-(–)-amino acid derivatives are considered as opposite sex pairs. Then their corresponding reaction products as enantiomer mixtures could be expected as natural behavior, which tends to occur in nature. This behavior looks like the sex selectivity of livings.
The discoveries of different chirality selectivities may disclose some secrets during life evolution. For example, if Lamino acids formed with a little more excessive quantity than D-amino acids due to some reasons in oceanic era, the excessive quantity of L-amino acids may be involved in life formation, then the left equal mole of L-and D-amino acids may take similar chirality pairing recognition reactions, because it is easy to form CHO-CHO and other simple organic compounds in oceanic era, the tiny acid under neutral conditions can accelerate the reactions. It may be a starting point of a new chemistry: evolution chemistry. Thus, it is possible to construct chirality pairing recognition or molecular sex recognition reaction which can be included in the range of evolution chemistry.
Experimental Section
General Experimental Procedures. Tryptophan methyl ester solution (1.0 mmol in 20 mL CH2Cl2) was cooled using ice-bath. The aldehyde (CHOCHO, 0.6 mmol) was then injected into the solution. Suitable molecular sieve was added to remove the water formed in Pictet-Spengler reaction. About 20 h later, 0.01 eq. of TFA was added into the solution for further cycloaddition. This procedure lasted over 40 h. Then the molecular sieve was filtered and the solution was removed under reduced pressure. The mixture was purified using chromatography column by silica gel. Ethyl acetate and petroleum ether mixture was used in the isolation. One major product 4a was obtained (60%), and the minor product (23%) was obtained. Total yield is 83%. Other products can be obtained under the similar procedure when starting materials changed to the corresponding compounds.
4a. ESIMS, m/z 459 [M + H]+. HRMS m/z calcd for C26H27N4O4 [M + H]+ 459.2032, found 459.2022. [α]D25 +156.3 (c 0.16, CHCl3). IR (KBr): 3379, 2949, 1736, 1608, 1486, 1466, 1452, 13371, 1199, 1022, 742 cm–1. 1H NMR (500 MHz, CDCl3) δ 2.08 (1H, br. s, NH-2'), 2.38 (1H, dd, J = 13.2, 9.0 Hz, H-4'), 2.50 (1H, dd, J = 13.2, 6.8 Hz, H-4'), 3.04 (1H, ddd, J = 15.7, 3.3, 1.6 Hz, H-4), 3.25 (1H, ddd, J = 15.6, 6.7, 1.6 Hz, H-4), 3.64 (3H, s, COOCH3-3'), 3.70 (3H, s, COOCH3-3), 3.90 (1H, dd, J = 9.0, 6.8 Hz, H-3'), 4.10 (1H, d, J = 5.5 Hz, H-1'), 4.27 (1H, dd, J = 6.6, 3.4 Hz, H-3), 4.58 (1H, br. s, NH-9'), 4.63 (1H, d, J = 5.5 Hz, H-1), 4.97 (1H, s, H-9'a), 6.63 (1H, d, J = 7.5 Hz, H-8'), 6.77 (1H, td, J = 7.5, 0.7 Hz, H-6'), 7.06– 7.12 (4H, m, H-5', 6, 7', 7), 7.29 (1H, d, J = 6.8 Hz, H-8), 7.46 (1H, d, J = 7.6 Hz, H-5), 8.32 (1H, br. s, NH-9). 13C NMR (125 MHz, CDCl3) δ 24.4 (C-4), 43.3 (C-4'), 52.0 (OCH3-3-3'), 52.2 (OCH3-3-3), 56.5 (C-3), 57.1 (C-1), 61.0 (C-3'), 64.7 (C-4'a), 72.2 (C-1'), 88.3 (C-9'a), 108.3 (C-4a), 109.5 (C-8'), 111.2 (C-8), 118.0 (C-5), 119.1 (C-6'), 119.4 (C-6), 121.8 (C-7), 123.3 (C-7'), 126.8 (C-5a), 128.5 (C-5'), 130.1 (C-5'a), 130.8 (C-9a), 136.5 (C-8a), 150.4 (C-8'a), 174.6 (CO-3'), 174.7 (CO-3).
4b. ESIMS, m/z 459 [M + H]+. HRMS m/z calcd for C26H27N4O4 [M + H]+ 459.2032, found 459.2046. [α]D25 –146.7 (c 0.15, CHCl3). IR (KBr): 3382, 2950, 2925, 1737, 1608, 1486, 1467, 1451, 1331, 1260, 1172, 1023, 742 cm–1. 1H NMR (500 MHz, CDCl3) δ 2.18 (1H, dd, J = 13.2, 7.7 Hz, H-4'), 2.20 (1H, br. s, NH-2'), 2.64 (1H, dd, J = 13.2, 7.5 Hz, H-4'), 3.07 (1H, dd, J = 13.2, 4.6 Hz, H-4), 3.23 (1H, dd, J = 13.2, 11.2 Hz, H-4), 3.66 (3H, s, COOCH3-3'), 3.86 (3H, s, COOCH3-3), 3.92 (1H, dd, J = 11.2, 4.6 Hz, H-3), 4.07 (1H, dd, J = 7.7, 7.5 Hz, H-3'), 4.32 (1H, d, J = 4.5 Hz, H-1), 4.43 (1H, br. s, NH-9'), 5.24 (1H, d, J = 4.5, 3.0 Hz, H-1'), 5.24 (1H, d, J = 3.0 Hz, H-9'a), 6.62 (1H, d, J = 7.5 Hz, H-8'), 6.79 (1H, dd, J = 7.0, 6.8 Hz, H-6'), 7.06 (1H, m, H-6), 7.07 (1H, m, H-7), 7.08 (1H, m, H-7'), 7.09 (1H, m, H-5'), 7.23 (1H, d, J = 7.7 Hz, H-8), 7.47(1H, m, H-5), 8.85 (1H, br. s, NH-9). 13C NMR (125 MHz, CDCl3) δ 24.9 (C-4), 43.7 (C-4'), 52.2 (OCH3-3-3'), 52.4 (OCH3-3-3), 58.1(C-3), 59.3 (C-1), 63.1 (C-3'), 63.8 (C-4'a), 72.6 (C-1'), 85.3 (C-9'a), 108.9 (C-4a), 109 (C-8'), 111.4 (C-8), 117.9 (C-5), 119.2 (C-6'), 119.4 (C-6), 121.7 (C-7), 122.7 (C-7'), 126.7 (C-5a), 128.5 (C-5'), 130.8 (C-9a), 131.5 (C-5'a), 136.6 (C-8a), 150.4 (C-8'a), 172.5 (CO-3'), 172.8 (CO-3).
11. ESIMS, m/z 343 [M + H]+. HRMS m/z calcd for C22H23N4 [M + H]+ 343.1922, found 343.1930. [α]D25 +42.6 (c 0.24, CHCl3). IR (KBr): 3382, 2920, 1672, 1609, 1486, 1468, 1337, 1178, 743 cm–1. 1H NMR (400 MHz, CDCl3) δ 2.02– 2.08 (1H, m, H-4'α), 2.27–2.34 (1H, m, H-4'β), 2.77–2.83 (1H, m, H-4β), 2.91–3.01 (4H, m, H-3α, 3', 4α), 3.27–3.33 (1H, m, H-3β), 3.86 (1H, d, J = 5.2 Hz, H-1'), 4.26 (1H, d, J = 5.1 Hz, H-1), 4.99 (1H, s, H-9'a), 6.56 (1H, d, J = 7.7 Hz, H-8'), 6.71 (1H, t, J = 7.3 Hz, H-6'), 6.99–7.07 (4H, m, H-6, 7, 5', 7'), 7.29 (1H, d, J = 7.8 Hz, H-8), 7.45 (1H, d, J = 7.6 Hz, H-5), 9.08 (1H, s, NH-9). 13C NMR (100 MHz, CDCl3) δ 21.1, 41.3, 43.5, 49.0, 58.0, 64.0, 72.9, 87.6, 109.4, 110.2, 111.4, 117.9, 119.2, 119.3, 121.5, 122.9, 127.1, 128.2, 131.7, 132.0, 136.5, 150.5.
12. ESIMS, m/z 371 [M + H]+. HRMS m/z calcd for C24H27N4 [M + H]+ 371.2235, found 371.2233. [α]D25 –269.8 (c 0.22, CHCl3). IR (KBr): 3428, 2918, 1608, 1470, 1336, 1188, 740 cm–1. 1H NMR (500 MHz, CDCl3) δ 1.21 (3H, d, J = 6.2 Hz, CH3-3), 1.44 (3H, d, J = 6.2 Hz, CH3-3'), 1.62 (1H, dd, J = 13.2, 11.2 Hz, H-4'α), 2.59–2.65 (2H, m, H-4α, 4'β), 2.90 (1H, ddd, J = 14.9, 4.0, 1.3 Hz, H-4β), 3.20–3.24 (1H, m, H-3), 3.42–3.47 (1H, m, H-3'), 3.83 (1H, d, J = 5.1 Hz, H-1'), 4.00 (1H, d, J = 3.4 Hz, NH-9'), 4.30 (1H, d, J =5.0 Hz, H-1), 5.24 (1H, d, J = 3.4 Hz, H-9'a), 6.65 (1H, d, J = 7.7 Hz, H-8'), 6.84 (1H, t, J = 7.3 Hz, H-6'), 7.05-7.12 (4H, m, H-6, 7, 5', 7'), 7.20 (1H, m, H-8), 7.44 (1H, m, H-5), 9.10 (1H, br. s, NH-9). 13C NMR (100 MHz, CDCl3) δ 18.9, 20.5, 31.2, 49.8, 51.2, 57.6, 58.4, 64.1, 75.2, 86.7, 109.1, 110.2, 111.4, 117.7, 119.1, 119.2, 121.4, 122.7, 126.7, 128.1, 131.2, 132.6, 136.5, 150.4.
13. ESIMS, m/z 487 [M + H]+. HRMS m/z calcd for C28H31N4O4 [M + H]+ 487.2345, found 487.2339. [α]D25 +115.2 (c 0.83, CHCl3). IR (KBr): 3394, 2924, 1736, 1608, 1465, 1237, 1035, 741 cm–1. 1H NMR (500 MHz, CDCl3) δ 1.91 (1H, dd, J = 12.8, 10.7 Hz, H-4'α), 2.03 (3H, s, H-OCH3-3), 2.04 (3H, s, H-OCH3-3), 2.20 (1H, dd, J = 15.7, 5.5 Hz, H-4'β), 2.87 (1H, d, J = 15.3 Hz, H-4β), 3.05 (1H, dd, J = 15.2, 7.1 Hz, H-4α), 3.22 1H, (m, H-3'), 3.72 (1H, m, H-3), 3.84 (1H, dd, J = 13.0, 10.8 Hz, H-3-CH2(α)OAc), 3.96 (1H, dd, J = 15.2, 6.5 Hz, H-3'-CH2(α)OAc), 4.04 (2H, m, H-1', 3'-CH2(β)OAc), 4.45 (1H, d, J = 5.3 Hz, H-1), 4.60 (1H, dd, J = 10.6, 4.4 Hz, H-3-CH2(β)OAc), 4.95 (1H, s, H-9'a), 6.64 (1H, d, J = 7.7 Hz, H-8'), 6.80 (1H, t, J = 7.8 Hz, H-6'), 7.08–7.20 (4H, m, H-6, 7, 5', 7'), 7.31 (1H, d, J = 8.0 Hz, H-8), 7.52 (1H, d, J = 7.8 Hz, H-5), 8.47 (1H, s, NH-9). 13C NMR (100 MHz, CDCl3) δ 20.8, 20.9, 22.3, 42.4, 51.3, 55.5, 58.2, 64.2, 64.5, 66.7, 71.6, 86.3, 108.8, 109.6, 111.1, 118.0, 119.1, 119.4, 121.9, 122.9, 127.5, 128.3, 130.5, 131.6, 136.7, 150.3, 170.9, 171.3.
14. ESIMS, m/z 611 [M + H]+. HRMS m/z calcd for C38H35N4O4 [M + H]+ 611.2658, found 611.2653. [α]D25 –166.7 (c 0.3, CHCl3). IR (KBr): 3384, 2919, 1719, 1605, 1466, 1272, 1112, 747, 710 cm–1. 1H NMR (500 MHz, CDCl3) δ 1.93 (1H, dd, J = 13.3, 10.5 Hz, H-4'α), 2.55 (1H, dd, J = 13.3, 6.3 Hz, H-4'β), 2.80 (1H, dd, J = 12.9, 8.7 Hz, H-4β), 3.00 (1H, dd, J = 15.1, 3.6 Hz, H-4α), 3.57 (1H, m, H-3'), 3.71 (1H, m, H-3), 3.78 (1H, d, J = 5.0 Hz, H-1'), 4.31–4.38 (3H, m, H-3-CH2(a)Obz, H-3'-CH2OBz), 4.69 (2H, m, H-1, H-3-CH2(b)OBz), 5.33 (1H, s, H-9'a), 6.47 (1H, d, J = 7.7 Hz, H-8'), 6.79 (1H, t, J = 7.4 Hz, H-6'), 7.03–7.07 (4H, m, H-6, 7, 5', 7'), 7.13 (1H, d, J = 7.4 Hz, H-8), 7.37 (2H, t, J = 7.7 Hz, HBz), 7.49–7.63 (5H, m, H-5, H-Bz), 7.90 (2H, d, J = 7.3 Hz, H-Bz), 8.08 (2H, d, J = 7.3 Hz, H-Bz), 8.72 (1H, s, NH-9). 13C NMR (100 MHz, CDCl3) δ 25.9, 45.2, 53.5, 58.2, 61.2, 64.2, 64.9, 67.9, 74.5, 87.1, 108.9, 109.0, 111.2, 117.8, 119.1, 119.3, 121.6, 122.7, 126.8, 128.2, 128.3, 128.8, 129.4, 129.5, 129.6, 129.8, 131.2, 132.5, 133.1, 133.5, 136.6, 150.3, 166.1, 166.2.
15. ESIMS, m/z 371 [M + H]+. HRMS m/z calcd for C24H27N4 [M + H]+ 371.2235, found 371.2234. [α]D25 +120 (c 0.13, CHCl3). IR (KBr): 3387, 2914, 1661, 1621, 1498, 1202, 1142, 803, 732 cm–1. 1H NMR (500 MHz, CDCl3) δ 2.18 (1H, m, H-4'α), 2.28 (3H, s, CH3), 2.40 (3H, s, CH3), 2.45 (1H, m, H-4β), 2.74 (2H, m, H-4), 2.92–3.23 (4H, m, H-3, 3'), 4.04 (1H, br. s, NH-9'), 4.18 (1H, s, H-1'), 4.22 (1H, s, H-1), 5.05 (1H, s, H-9'a), 6.51 (1H, d, J = 8.7 Hz, H-8'), 6.93–7.18 (5H, m, H-Ar), 9.86 (1H, s, NH-9). 13C NMR (100 MHz, CDCl3) δ 20.9, 21.6, 21.8, 37.5, 43.3, 47.1, 56.5, 63.2, 70.5, 88.2, 109.8, 111.3, 111.6, 117.9, 123.8, 124.1, 127.1, 127.4, 128.6, 128.8, 129.5, 130.1, 135.3, 148.7.
22. Racemic mixture was resolved by HPLC on a Chiralcell-OD-H column (250 × 10 mm, n-hexane/i-PrOH = 85/15). [α] 25 D +187.3 (c 0.12, CHCl3). ESIMS, m/z 459 [M + H]+. HRMS m/z calcd for C26H27N4O4, [M + H]+ 459.2032, found 459.2026. 1H NMR (500 MHz, CDCl3) δ 2.28 (1H, dd, J = 13.5, 7.7 Hz, H-4'α), 2.68 (1H, dd, J = 13.5, 7.7 Hz, H-4'β), 3.07 (1H, d, J = 15.7 Hz, H-4β), 3.32 (1H, dd, J = 15.6, 7.1 Hz, H-4α), 3.64 (3H, s, COOCH3-3), 3.66 (3H, s, COOCH3-3'), 3.84 (1H, d, J = 4.5 Hz, H-1'), 4.00 (1H, t, J = 7.8 Hz, H-3'), 4.28 (1H, d, J = 6.7 Hz, H-3), 4.50 (1H, br. s, NH-9'), 4.70 (1H, d, J = 3.7 Hz, H-1), 5.06 (1H, s, H-9'a), 6.60 (1H, d, J = 7.8 Hz, H-8'), 6.80 (1H, t, J = 7.4 Hz, H-6'), 7.08–7.12 (4H, m, H-6, 7, 5', 7'), 7.21 (1H, d, J = 7.9 Hz, H-8), 7.48 (1H, d, J = 7.0 Hz, H-5), 8.48 (1H, s, NH-9). 13C NMR (125 MHz, CDCl3) δ 24.5, 44.3, 51.9, 52.2, 55.2, 56.2, 62.4, 64.4, 74.1, 88.2, 108.3, 109.5, 111.2, 117.9, 119.1, 119.3, 121.7, 122.8, 126.9, 128.5, 130.4, 131.0, 136.6, 150.7, 173.0, 174.8. The ent-22 had the –189.2 of optical rotation values in chloroform.
23. Racemic mixture was resolved by HPLC on a Chiralcell-OD-H column (250 × 10 mm, n-hexane/i-PrOH = 87/17). [α]D25 +150.3 (c 0.45, CHCl3). ESIMS, m/z 487 [M + H]+. HRMS m/z calcd for C28H31N4O4, [M + H]+ 487.2345, found 487.2364. 1H NMR (500 MHz, CDCl3) δ 1.62 (1H, dd, J = 12.9, 10.6 Hz, H-4'α), 1.95 (3H, s, OCOCH3-3-3'), 2.07 (3H, s, OCOCH3-3-3), 2.35 (1H, dd, J = 16.5, 8.0 Hz, H-4'β), 2.90 (1H, d, J = 15.6 Hz, H-4β), 3.08 (1H, dd, J = 15.7, 7.5 Hz, H-4α), 3.73 (2H, m, H-3, 3'), 3.88 (2H, m, H-1', CH2(α)OAc-3), 4.03 (2H, m, CH2OAc-3'), 4.45 (1H, d, J = 5.2 Hz, H-1), 4.52 (1H, br. s, NH-9'), 4.72 (1H, dd, J = 10.7, 3.7 Hz, CH2(β)OAc-3), 5.09 (1H, s, H-9'a), 6.63 (1H, d, J = 7.8 Hz, H-8'), 6.78 (1H, t, J = 7.4 Hz, H-6'), 7.06–7.12 (4H, m, H-6, 7, 5', 7'), 7.24 (1H, d, J = 6.7 Hz, H-8), 7.51 (1H, d, J = 6.7 Hz, H-5), 8.78 (1H, s, NH-9). 13C NMR (125 MHz, CDCl3) δ 20.8, 21.0, 23.3, 44.4, 52.0, 53.8, 60.8, 63.7, 64.4, 66.0, 74.4, 88.0, 108.2, 109.4, 111.3, 117.9, 119.1, 119.2, 121.7, 122.7, 127.4, 128.2, 130.6, 132.9, 136.6, 150.1, 170.9, 171.3. The ent-23 had the –148.5 of optical rotation values in chloroform.
24. Racemic mixture was resolved by HPLC on a Chiralcell-OD-H column (250 × 10 mm, n-hexane/i-PrOH = 85/15). [α] 25 D +127.6 (c 0.47, CHCl3). ESIMS, m/z 611 [M + H]+. HRMS m/z calcd for C38H35N4O4, [M + H]+ 611.2658, found 611.2650. 1H NMR (500 MHz, CDCl3) δ 1.84 (1 H, dd, J = 12.9, 10.5 Hz, H-4'α), 2.44 (1H, dd, J = 13.1, 6.3 Hz, H-4'β), 3.02 (1H, d, J = 15.5 Hz, H-4β), 3.16 (1H, dd, J = 16.1, 7.0 Hz, H-4α), 3.84 (1H, m, H-3'), 3.92 (2H, m, H-3, 1'), 4.14 (1H, dd, J = 10.5, 8.7 Hz, CH2(a)OBz-3), 4.27 (1H, dd, J = 14.5, 3.9 Hz, CH2(a)OBz-3'), 4.44 (1H, dd, J = 14.5, 7.0 Hz, CH2(b)OBz-3'), 4.54 (1H, d, H-1), 4.58 (1H, br. s, NH-9'), 5.01 (1H, dd, J = 13.5, 4.5 Hz, CH2(b)OBz-3), 5.20 (1H, s, H-9'a), 6.58 (1H, d, J = 7.8 Hz, H-8'), 6.79 (1H, t, J = 8.7 Hz, H-6'), 7.07-7.15 (4H, m, H-6, 7, 5', 7'), 7.28 (1H, m, H-8), 7.39 (2H, t, J = 7.7 Hz, H-Bz), 7.44 (2H, t, J = 7.7 Hz, H-Bz), 7.51–7.59 (3H, m, H-5, H-Bz), 7.93 (2H, d, J = 7.5 Hz, H-Bz), 7.98 (2H, d, J = 7.5 Hz, H-Bz), 8.86 (1H, s, NH-9). 13C NMR (125 MHz, CDCl3) δ 23.7, 44.5, 52.2, 53.9, 60.9, 64.0, 65.0, 65.6, 74.5, 88.4, 108.4, 109.5, 111.3, 117.9, 119.1, 119.2, 121.8, 122.7, 127.4, 128.2, 128.3, 128.4, 129.5, 129.6, 129.8, 130.1, 130.6, 130.9, 132.8, 133.1, 136.7, 150.2, 166.2, 166.8. The ent-24 had the –130.2 of optical rotation values in chloroform.
25. Racemic mixture was separated by HPLC on a Chiralcell-OD-H column (250 × 10 mm, n-hexane/i-PrOH = 92/8). [α] 25 D +166.7 (c 0.12, CHCl3). ESIMS, m/z 371 [M + H]+. HRMS m/z calcd for C24H27N4, [M + H]+ 371.2235, found 371.2238. 1H NMR (600 MHz, CDCl3) δ 1.13 (3H, d, J = 6.3 Hz, CH3-3), 1.25 (3H, d, J = 6.6 Hz, CH3-3'), 1.43 (1H, dd, J = 12.6, 10.8 Hz, H-4'α), 2.44 (1H, dd, J = 13.6, 5.6 Hz, H-4'β), 2.59 (1H, d, J = 15.5 Hz, H-4α, ), 3.16 (1H, dd, J = 15.0, 5.9 Hz, H-4β), 3.46 (1H, m, H-3'), 3.76 (1H, m, H-3), 3.82 (1H, d, J = 5.7 Hz, H-1'), 4.07 (1H, br. s, NH-9'), 4.43 (1H, d, J = 5.6 Hz, H-1), 5.07 (1H, s, H-9'a), 6.59 (1H, d, J = 7.7 Hz, H-8'), 6.76 (1H, t, J = 7.5 Hz, H-6'), 7.04–7.11 (4H, m, H-6, 7, 5', 7'), 7.18 (1H, dd, J = 6.1, 2.8 Hz, H-8), 7.48 (1H, dd, J = 6.4, 2.6 Hz, H-5), 8.93 (1H, br. s, NH-9). 13C NMR (150 MHz, CDCl3) δ 16.8, 19.6, 28.7, 48.5, 51.3, 52.5, 58.3, 64.7, 74.9, 88.9, 108.9, 109.1, 111.2, 117.8, 118.9, 119.0, 121.4, 122.7, 127.7, 127.9, 130.9, 133.5, 136.6, 150.3. The ent-25 had the –170.3 of optical rotation values in chloroform..
Notes
Electronic Supplementary Material
Supplementary material is available in the online version of this article at http://dx.doi.org/10.1007/s13659-012-0003-6 and is accessible for authorized users.
Acknowledgments
Hua-Jie Zhu thanks the financial supports from NSFC (30873141), 973 Program (2009CB522300), Hebei University and the State Key Laboratory of Phytochemistry and Plant Resources in West China.
References
-
1.(a) Rich, A. J. Biol. Chem. 2006, 281, 7693–7696. (b) Forsdyke, D. R. J. Theo. Biology. 2007, 249, 325–330. PubMed Google Scholar
-
2.T. Ikai, Y. Okamoto, Chem. Rev. 109, 6077-6101 (2009) CrossRef PubMed Google Scholar
-
3.B. Bai, L. Shen, J. Ren, H.J Zhu, Adv. Syn. & Cat. (2011) PubMed Google Scholar
-
4.F. R. Bou-Hamdan, J. L. Leighton, Angew. Chem. Int. Ed. 48, 2403-2406 (2009) CrossRef PubMed Google Scholar
-
5.(a) Intermediate 2 reacted with 1 formed only (E)-imines since thecalculated energy of formation of the (Z)-imine is 5.1 kcal/molhigher than that for the (E)-imine in CH2Cl2. Two products wereisolated in ratios of 2: 1 to 3: 1 after subsequent reactions with trans-2 and cis-2 (theoretically 4: 1 at the B3LYP/6-31G(d) level), respectively. (b) Nyerges, M.; Rudas, M.; Bitter, I.; Toke, L. Tetrahedron 1997, 53, 3269–3280. (c) Gomez-Monterrey, I. M.; Campiglia, P.; Bertamino, A.; Aquino, C.; Mazzoni, O.; Diurno, M. V.; Iacovino, R.; Saviano M.; Sala, M.; Novellino, E.; Grieco, P. Eur. J. Org. Chem. 2008, 1983–1992. (d) Hili, R.; Yudin, A. K. J. Am. Chem. Soc., 2006, 128, 14772–14773. PubMed Google Scholar
Copyright information
© The Author(s) 2012
Open Access
This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.